WebMay 19, 2024 · 1. Introduction. Peach (Prunus persica L. Batsch) is economically a very important fruit crop in Korea.Generally, people eat it as a table fruit. In Korea, it is used in different food industries for making jam (Boksoongah jam), jelly (jelly in jelly), tea (Boksoongah hong Chaa), and juice (Kolpeach).Addition of peach fruit could increase the … WebThe total RNA was converted to cDNA with a cDNA synthesis kit (BIOFACT, Korea). Further, SYBR Green (BIOFACT, Korea) was used to specify the expression of the intended genes (Beyrami et al. Citation 2024). The genes were amplified as follows: initial denaturation at 95 °C for 5 min (1X), followed by 35 cycles of 95 °C for 30 s, primer ...
2024 103:200-213 https://doi.org/10.1094/PDIS-05-18-0857 …
WebNov 12, 2024 · sgRNAs were digested in vitro using the Cas9 protein (Toolgen, Korea). The target substrate was PCR-amplified using the primers 5′-CTTCTCCTTCTTCACGCGC-3′ and 5′-CTCTGGCCTTGGTATGTCCA-3′, and the PCR product was purified using the HiGene Gel & PCR purification system (BioFACT, Korea). ... (BioFACT, Korea). The … WebMar 15, 2024 · K. brevifasciata was collected from the Sinpyeongcheon Stream under a collection permit issued by the Ministry of Environment in Korea. K. brevifasciata genomic DNA was extracted from the caudal fin using the Genomic DNA Prep Kit (Biofact, Korea), and the extracted genomic DNA was stored at Soonchunhyang University. The complete … mary s shoemaker school
:: JNH :: Journal of Nutrition and Health
WebMar 29, 2024 · Clostridium difficile is one of the main etiological agents causing antibiotic-associated diarrhea. This study investigated the genetic diversity of 70 toxigenic C. difficile isolates from two Korean hospitals by employing toxinotyping, ribotyping, multilocus sequence typing (MLST), and pulsed-field gel electrophoresis (PFGE). Toxin gene … WebOct 29, 2024 · According to the manufacturer's protocol, cDNA synthesis was performed using a BioFACT RT-Kit (Biofact, Korea) and stored at − 20 °C until the next step. Quantitative real-time PCR (qPCR) Real-time PCR were performed using SYBR Green master mix (Biofact. Korea) and specific primers conducted on Rotor-Gene 6000 … WebBioFact™ 2X OneStep Multiplex qRT-PCR Kit for Probe is the best optimized to a system which is using sequence specific designed probe from various RNA samples. The … hutch\\u0027s chimney service